FIREScript® RT cDNA synthesis MIX
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C. FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Reagents FIREScript® Enzyme Mix (FIREScript® RT and RiboGrip RNase inhibitor) 10x RT Reaction Premix without primers RT reaction buffer with DTT and dNTPs Water, nuclease free